Fig 1: Identification of SP1 as the mutual transcription factor of MRPs and 12LOX. a Bioinformatic analysis of transcription factors of MRP1, MRP4, Snail, Slug, 12LOX, COX1, and CYP2J2. b SP1 binding sequences in the promoters of MRP1 (ABCC1), MRP4 (ABCC4), and 12LOX (ALOX12). c ChIP assay of SP1 binding on the promoters of MRP1 (ABCC1), MRP4 (ABCC4), and 12LOX (ALOX12). d, e The mRNA (d) and protein (e) expression levels of SP1 in SKOV3 and SKOV3-R cells. Data in (d) are expressed as mean ± SEM of 4 independent experiments. ***P < 0.001. f The unpaired Student’s t test was used to analyze the expression of SP1 in the GEO database (GSE114206). Data are expressed as mean ± SEM and *P < 0.05. g Patient survival data were obtained from TCGA database and overall survival probability was then calculated using the Kaplan-Meier method and the differences in survival curves were analyzed using the log-rank test. P-values of 0.05 or less were considered significant
Fig 2: Immunofluorescence staining for ALOX12 and infiltrated CD4(+)-T cells and CD14(+)-monocytes in atherosclerotic plaque and non-plaque intima of common carotid artery of a 54-year-old man. White arrow, cells co-stained with CD14 and ALOX12 antibodies
Fig 3: Promoter CpG islands and regions for the pyrosequencing of the five target genes identified after methylation-specific PCR (a) and methylation levels of the genes in plaques (P) and non-plaque (NP) intima of common carotid arteries in 20 cadavers (b). Correlation between promoter methylation and expression of AIRE1 (c) and ALOX12 (d) in 18 carotid endarterectomy plaques. Open bar, exon 1 region; closed bar, the regions targeted for bisulfite pyrosequencing; open bar in the middle of closed bar; sequencing region, arrow; transcriptional start site of each gene, *p < 0.05 on paired t test
Supplier Page from Abcam for Anti-12 Lipoxygenase/ALOX12 antibody